INVESTIGADORES
LAJMANOVICH Rafael Carlos
artículos
Título:
Tissue-specific variations of esterase activities in the tadpoles and adults of Pseudis paradoxa (Anura: Hylidae).
Autor/es:
ATTADEMO A.M.,; PELTZER P.M; LAJMANOVICH R.C; BASSÓ A.; JUNGES C.M
Revista:
WATER AIR AND SOIL POLLUTION
Editorial:
SPRINGER
Referencias:
Lugar: Berlin; Año: 2014 p. 1903 - 1910
ISSN:
0049-6979
Resumen:
We determined basal levels of cholinesterase(ChE) and carboxylesterase (CbEs; two substrates: α-naphthyl acetate and 4-nitrophenylvalerate) in differenttissues of tadpoles and adults of the frog Pseudisparadoxa and evaluated their use as complementarybiomarkers of anti-cholinesterase pesticide exposure.ChE and CbEs sensitivity to malaoxon was alsoevaluated. Adults and tadpoles were collected withsweep net from temporary ponds located in naturalriparian forests along the Paraná River (GarayDepartment, Santa Fe province, Argentina). Wefound significant differences in B-esterase activitiesbetween adults and tadpoles and among differenttissues. The in vitro inhibition tests indicated thatChE is more sensitive to inhibition than CbEs. Ourresults suggest that basal ChE and CbE (α-NA and 4-NPV) activities in different tissues of adult and tadpolesP. paradoxa would be suitable biomarkers ofpesticide exposure, and this amphibian species couldbe used as sentinel in field monitoring.
