INEU   29757
INSTITUTO DE NEUROCIENCIAS
Unidad Ejecutora - UE
artículos
Título:
Protocol for morphometric analysis of neurons derived from human pluripotent stem cells
Autor/es:
RODRIGUEZ VARELA MS; SEVLEVER GE; ISAJA L; SCASSA ME; MUCCI S; FERRIOL-LAFFOUILLERE, SOFÍA LUJÁN; ROMORINI L
Revista:
STAR protocols
Editorial:
ELSEVIER
Referencias:
Año: 2022
ISSN:
2666-1667
Resumen:
The analysis of morphological features of neurons derived from human pluripotent stem cells (hPSCs) is important to describe neuronal phenotypes and changes observed throughout development. Using free and easily accessible tools, we describe a protocol for the morphometric quantification of hPSCs-derived neurons in two- and three-dimensions in vitro cultures. We detail the analysis of soma area and main and secondary dendrites lengths of GFP-transfected neurons and the measurement of area and perimeter of immunostained neurospheres.