INVESTIGADORES
ZAMPINI Iris Catiana
congresos y reuniones científicas
Título:
IN VITRO EVALUATION OF ANTIGENOTOXIC ACTIVITY OF CASSIA APHYLLA EXTRACTS USING HEP G2 CELLS
Autor/es:
ZAMPINI IC, JIMBUEM YAO S2, VILLARINI M, MORETTI M, ISLA MP.
Lugar:
Tafí del Valle - Tucumán
Reunión:
Jornada; Jornadas de Biología; 2006
Resumen:
Cassia aphylla Cavo (Leguminosae), is a endemic plant specie dis­tributed in the westem Argentina. This specie is known under the common names of pichanilla or cabello de indio and traditionally used for the cough and as antiseptic or healing. In a previous work we reported the antimicrobial activity of ethanolic extract of this plant specie against multi-resistant Gram-negative human patho­genic bacteria. The aim ofthe present study was to evaluate the antigenotoxic effect of Cassia aphylla ethanolic extract using the alkaline Comet assay on metabolically competent human-derived hepatoma (Hep G2) cells. The result showed that C. aphylla extract was able to diminish the DNA damage induced by benzo[ a ]pyrene (B [a ]P) (indirect mutagen or promutagen) but did not present antigenotoxic activity against 4-NQO (direct mutagen). Otherwise, the assayed extract did show a weak genotoxic effect on Rep G2 cells in the first 4h of treat­ment, but no genotoxic effect was observed after 24 h. Although the antigenotóxic effect of the extract of Cassia are evident, is nec­essary to characterize chemically to the metabolite responsible for the effect before suggesting its use like phytotherapic product.