A NEW AUTOGRAPHIC METHOD FOR DETERMINATION OF LIPOPHYLIC AND HYDROPHILIC ANTIOXIDANT COMPOUNDS

ZAMPINI IRIS C.; MESÓN GANA JIMENA; ISLA MARÍA INÉS

Tafí del Valle - Tucumán

Jornada; Jornadas de Biología; 2005

Several classes of dietary compounds have been suggested to reduce the level of biomarkers of oxidative damage. The antioxidant actions may occur by inhibition of reactive oxygen species generation or by its direct scavenging. Simple experiments can be performed to examine direct antioxidant ability in vitro. This work describe a new autographic method to detect lipophilic and hydrophilic antioxidant components in food, phytoterapic or other mixtures using a preformed radical monocation of 2,2 azinobis-(3- ethylbenzothia-zoline-6-sulfonic acid) (ABTS·+).  Different concentrations of reference antioxidant compounds seeded in  TLC were covered with agar containing ABTS·+ or sprayed  with  an ABTS·+ solution. The detection limit was 0.1mg for naringenin and quercetine, 1 mg for rutine and b-carotene, 2.5 mg for ascorbic acid and  0.15 mg of phenolic compounds from plant extracts. This method is rapid, sensitive and reproducible for screening of complex mixtures with antioxidant properties and for the bioguide isolation of antioxidant metabolites.