ANTIOXIDANT ACTIVITY OF A CHALCONE ISOLATED FROM AMAICHA DEL VALLE PROPOLIS, TUCUMAN

NIEVA MORENO M.I.; ZAMPINI I. C; ORDOÑEZ R.M; VATTUONE M; ISLA M. I.

Tafí del Valle - Tucumán

Jornada; Jornadas de Biología; 2004

We have previously demonstrated that North Argentine propolis exhibit protective action against oxidative modification of lipid and reactive oxygen species scavenging activities (ROS). These results justify the use of propolis as a possible source of natural antioxidants. Recently, we isolated from Amaicha del Valle propolis a antibacterial compound that was identified as 2’,4’ dihydroxychalcone. The purpose of this work was to compare the antioxidant activities between propolis extract and the bioactive compound using the b-carotene-linoleic acid assay. The Amaicha del Valle propolis can hinder the extent of b-carotene-bleaching by neutralising the linoleate-free radical and other free radicals formed in the system. The IC50 (flavonoid concentration required for exerting the 50 % of protective activity) was 0.4 µg/ml. The TLC chromatograms revealed with ß-carotene-linoleate system, also confirm the antioxidant activity of the antibiotic compound. Its antioxidant activity is similar to quercetin, a powerfull natural antioxidant (0.3 µg/ml). Our results suggest a potential use of Amaicha del Valle propolis as phytopharmaceutic products and for food conservation.