INVESTIGADORES
ARGAÑARAZ Martin Eduardo
artículos
Título:
Use of biochemical and protein profiles of seminal plasma to prediction of semen quality and fertility in stallions
Autor/es:
CALOGERO STELLETTA; SILVIA ALBERTI; BESTE ÇIL; KORAY TEKIN; MEHMET BORGA TIRPAN; MARTIN E. ARGAÑARAZ; ERGUN AKCAY; ALI DASKIN
Revista:
POLISH JOURNAL OF VETERINARY SCIENCES
Editorial:
POLISH ACAD SCIENCES
Referencias:
Lugar: Bialowieza; Año: 2021 vol. 24 p. 505 - 514
ISSN:
1505-1773
Resumen:
The identification of various substances in seminal plasma has opened the way to study theirfunctionality. It was aimed to identify the electrophoretic protein profile (EPP) and biochemicalparameters (BP) of seminal plasma (SP) as predictors of semen quality and fertility in stallion.Forty-six ejaculates from 7 fertile stallions, aged between 6-26 years, were collected from Mayto July and 117 mares were used to obtain fertility data. For each ejaculate, volume, sperm motility, concentration were determined and seminal plasma samples were collected to perform one--dimensional electrophoresis and biochemical profiling. Following the estrus detection, mareswere inseminated with fresh sperm. Pregnancy rates and foal rates were recorded. The concentration of 15-18 kDa molecular weight (MW) proteins has shown a positive correlation with sperm concentration and foal rate. Besides, a strong positive correlation was found between sperm concentration and 23-28 kDa MW proteins (r=0.77). The volume of 19-22 kDa MW proteins wasnegatively correlated with pregnancy and foal rate. Similarly, the volume of high MW proteins(173-385 kDa) correlated negatively with sperm motility and foal rate. Apart from the proteinprofile, while Magnesium and Glucose levels were negatively correlated with sperm quality andfoal rate, Cholesterol level was a positive indicator of the quality of semen as well as the foalingrate. Moreover, the total protein level was correlated negatively with the sperm concentrationwhereas triglyceride was correlated positively. In conclusion, EPP and BP of seminal plasma arevaluable clinical tools as predictors of fertility and semen quality in the stallion