Differential effects of retinoic acid plus n-9 unsaturated fatty acids on two human breast cancer lines

QUIROGA PL; SORIA EA; VALENTICH MA; EYNARD AR

Ginebra (Suiza)

Congreso; UICC Congress; 2008

Background: All-trans retinoic acid (RA) and unsaturated fatty acids (UFAs) exhibit different effects on tumor parameters such as proliferation and differentiation. Although both varieties of nutrients per se may play a modulating role on the carcinogenic process (De Antueno et al., 1997; Hansen et al., 2000), the combined use of n-9 UFAs plus RA has not been fully studied. Objectives: The purpose of this work was to modulate certain tumoral parameters related to cancer differentiation on two mammary cancer cell lines (ZR-75-1 and MCF-7) by RA, both with and without n-9 UFAs. Methods: At 90% confluence, cells were incubated with RA 1 µM or RA plus n-9 UFAs 50 µM: oleic acid (OA) or eicosatrienoic acid (n-9, ETA), an unusual UFA related to EFA (Essential Fatty Acids) deficiency and cancer (Eynard et al. 1998). The lipid profile (GLC), g-glutamyltranspeptidase activity (GGTP, Szasz method), E-cadherin and actin expression (immunocytochemistry) were assessed. Statistics: ANOVA + LSD Fisher test (p