Arachis hypogaea L. tegument ethanolic extract modulates innate immune response in a model of infection with dengue virus

SABINI MC; CODEMO CA; PORTELA MA; PEÑA VS; SUÁREZ PERRONE A; PERALTA MI; SORIA EA

Ciudad Autónoma de Buenos Aires

Congreso; 14° Latin American and Caribbean Immunology Congress; 2024

ALACI

In many infectious and non-infectious diseases, the immune response is deregulated. In viral infections such as dengue, the production of certain cytokines can be exacerbated, contributing to immunopathogenesis. Arachis hypogaea L. -peanut- has numerous medicinal properties. Therefore, peanut extract could regulate the host´s immune response, improving its immune status and preventing diseases.The aim of this work was to evaluate cytotoxicity in human mononuclear cells and the immunomodulatory effect on pro-inflammatory and anti-inflammatory cytokines of the tegument ethanolic extract of peanut (TEE), in a model of infection with dengue virus serotype 2 (DENV2).The EET was obtained by simple alcoholic extraction method. For cytotoxicity and immunomodulation assays, peripheral blood mononuclear cells (PBMCs) from healthy human donors (Banco de Sangre, UNC) were used. Cells were obtained with Ficoll-Hypaque, seeded in culture plates with RPMI-1640 medium, and treated with TEE at concentrations from 50 to 200 µg/mL and incubated at 37°C for 48 h. Cell viability was determined by trypan blue exclusion.For immunomodulation, PBMCs were stimulated with DENV2 (50 PFU/mL) and treated with TEE at 10 and 20 µg/mL. Cellular (CC) and viral (VC) controls were included. After 48 h, supernatants were removed, centrifuged, and TNF-α, IL-6, and IL-10 production was assessed using ELISA kits. Statistical analysis was performed using GraphPadPrism 6.0.TEE cytotoxicity studies indicated a viability greater than 80% at concentrations ≤200µg/mL. Immunomodulation results revealed a significant increase (p