Ex vivo skin penetration and in vivo toxicity of nanoberries for topical delivery of antioxidants

PAULA BUCCI; NATALIA CALIENNI; JIMENA PRIETO; SILVIA ALONSO; JORGE MONTANARI

Estambul

Congreso; Nanobiotechnology Days 2015 ? Workshops/Lectures; 2015

Istanbul Kemerburgaz University

Purpose: To evaluate the skin penetration ex vivo of novel Nanoberries (NB), ultradeformable liposomes for skin delivery of blueberry extract rich in polyphenols, and to assess their in vivo nanotoxicity.Materials and Methods: NB were prepared by resuspension in a blueberry ethanolic extract solution of a lipid film (soy phosphatidylcholine and sodium cholate as border activator) followed by size and lamellarity reduction by sonication. Human skin explants were obtained from surgery discards and NB with double fluorescent labeling were non-occlusively applied on a Saarbrücken Penetration Model device. Blueberry extract was quantified in the stratum corneum (SC) after tape stripping. Skin slices were studied by confocal microscopy. Lethal dose and swimming activity were determined in Zebrafish (Danio rerio) larvae model.Results: NB were about 100 nm in diameter, with low polydispersity. Skin penetration profiles showed that the extract reached deeper layers when applied into NB in comparison to conventional liposomes or in its free form. Confocal microscopy images are in concordance with penetration profiles. NB were non toxic at the concentration used for skin penetration.Conclusion: NB effectively entered the skin through the SC, with low toxicity. We are presently conducting photoprotection studies in vitro in order to determine the ability of the NB to lower the phototoxicity triggered by exposure to UV rays. NB could become a potential topical treatment against ageing and damage of the skin.