Lipid-polymer membranes as carrier for L-tryptophan: biophysics and metabolic properties

FERNANDEZ RUOCCO, MARIA JULIETA; SIRI, MACARENA; MAROTTA HERNAN; PRIETO MARIA JIMENA; ALONSO, SILVIA DEL VALLE; CHIARAMONI, NADIA

Congreso; Reunion Sociedad Argentina de Biofisica; 2012

Sociedad Argentina de Biofisica

A study of polymeric liposomes encapsulating L-tryptophan was carried out with the main goal of characterizing them as drug delivery systems.Polymeric liposomes were obtained by UV irradiation of vesicles with 1,2-bis(10,12- tricosadiynoyl)-sn-glycero-3-phosphocholine (DC8,9PC) and 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) in 1:1 molar ratio, and also obtained in presence of 10 and 50 mol % of L-tryptophan (respect to total lipid concentration).  Polymerization efficiency was studied spectrofotometrically. Also, bilayer packing at  the polar head  region was  followed with  the probe Merocyanine 540  (MC540) and  interactions between lipopolymer and L-tryptophan were studied by FTIR.