INVESTIGADORES
CATALANO Santiago Andres
congresos y reuniones científicas
Título:
Clonado de un gen de la familia â-glucosidasa expresada en el tronco de nogal (Juglans L.)
Autor/es:
CATALANO, SA; BRETON, C
Lugar:
Trelew-Chubut
Reunión:
Jornada; XXXIII Congreso Argentino de Genética; 2005
Resumen:
Several tree species presents in its trunk two regions clearly differentiated, heartwood and sapwood. The heartwood is important. Previous analyses showed that HJG would be a major precursor of HW colour formation and that a B-glucosidase would be related to the last steps in its degradation in colour compounds. Therefore we inteded to clone the gene expressed in Juglans wood by screening two different cDNA libraries fagos one from the limit betweeen HW and SapW and the other from cambium. Several clones were isolated in TZ and a posteriori sequenced. Blast search indicated that the sequences cloned were members of BG 1 family. Two different variants were obtained from YW library and one from TZ. The sequence in not only in non coding 3 prime end region but also in the deduced Amino Acid sequence. Phylogeny analysis  indicate that this sequence were more related to ther BG isolated from walnut fruit that to others sequences of bg of different species.