Development and characterization of microsatellite loci in the entocommensal Malacobdella arrokeana (Nemertea: Bdellonemertea), from Patagonia (Argentina) and crossamplification in 34 nemertean species

ALFAYA J.E.F.; LOPEZ-MÁRQUEZ V.; GARCıA-JIMENEZ R.; FERNANDEZ-ALVAREZ F.A; BIGATTI G.; MACHORDOM, A.

Conservation Genetic Resources

SPRINGER

Lugar: Berlin; Año: 2014 vol. 6 p. 147 - 150

Microsatellite loci (26 in total) were isolatedfor the first time for the entocommensal nemertean Malacobdellaarrokeana, using 454 GS-FLX Titanium pyrosequencing.We developed conditions for amplifying thesemarkers in 8 multiplex and 5 individual reactions. One to14 alleles were detected per locus across 25 samples analyzedfrom San Matı´as Gulf, Patagonia (Argentina). For the25 polymorphic loci, observed and expected heterozygositiesranged from 0.125 to 0.920 and 0.119 to 0.890,respectively; five loci deviated from Hardy?Weinbergequilibrium. Limited distribution and host specificity of M.arrokeana, which only inhabits Panopea abbreviata, haveendangered this nemertean given the unregulated commercialexploitation of its host. These useful markersprovide data for future conservation strategies. Crossamplificationswere also tested in 34 nemerteans species,representing the major lineages in this phylum. In total, 18of the 26 loci (from one to 11 per species) gave clear allelicprofiles.