INVESTIGADORES
CHOI Marcelo Roberto
congresos y reuniones científicas
Título:
Renal dopaminergic system dysfunction and its association with arterial hypertension and renal inflammation in an experimental model of metabolic syndrome induced by fructose overload
Autor/es:
RUKAVINA MIKUSIC NL; KOUYOUMDZIAN NM; KRAVETZ MC; ROUVIER E; ROBBESAUL GD; ÁLVAREZ PRIMO M; DEL MAURO J; LEE HJ; PUYÓ AM; TOBLLI JE; FERNÁNDEZ BE; CHOI MR
Lugar:
Mar del Plata
Reunión:
Congreso; LXI Reunión Anual de la Sociedad Argentina de Investigación Clínica (SAIC); 2016
Institución organizadora:
Sociedad Argentina de Investigación Clínica (SAIC)
Resumen:
Introduction: Renal dopaminergic system (RDS) promotes sodium excretion and anti-inflammatory actions. A high fructose diet induces metabolic and hemodynamic changes that could be associated with an impairment of the RDS, leading to renal inflammation, sodium retention and arterial hypertension. Aim: to evaluate RDS state and its association with the development of hypertension and overexpression of renal inflammatory markers in fructose overloaded (FO) rats. Methods: Male Sprague Dawley rats were assigned to Control (C, tap water) or FO (10% w/v of fructose solution) groups, during 4, 8 and 12 weeks (n=8/group/period). Urinary L-dopa and dopamine (DA), diuresis and albuminuria were determined. Systolic blood pressure (SBP) and metabolic parameters were measured. Western blot analysis of renal expression of D1R, NFκβ, IL-6, TNF-α, TGF-β1 and nephrin were performed. Results: FO increased SBP (mmHg, C4: 121±8 vs. F4: 145±1*; C8: 130±4 vs. F8: 161±10#; C12: 133±5 vs. F12: 163±4#), which positively correlated (R2=0.78; p<0.002) to urinary L-dopa/DA index (C4: 0.49±0.05 vs. F4: 1.9±0.09#; C8: 0.53±0.06 vs. F8: 2.35±0.1#; C12: 0.54±0.07 vs. F12: 2.57±0.2#). A significant decrease of D1R expression was accompanied by a significant increase in nFκβ, IL-6, TNF-α, TGF-β1 expression since week 4. Microalbuminuria (C12:13.11±1.4 vs F12:57.6±2.5#) and a significant decrease in nephrin expression (C12: 1.00±0.10 vs. F12: 0.73±0.05#) were only observed at week 12. (*p<0.05, #p<0.01 vs. C). Conclusion: FO was associated with an increased L-dopa/DA index and decreased D1R expression since week 4 of treatment. The RDS dysfunction was accompanied by an increase in blood pressure levels and renal expression of inflammatory markers in all experimental periods. Alteration of L-dopa/DA index could be postulated as an earlier marker of renal dysfunction than structural damage evidenced by microalbuminuria and decreased nephrin expression in week 12 of FO.