INVESTIGADORES
CHOI Marcelo Roberto
artículos
Título:
Reduction of eNOS in Vascular Smooth Muscle by Salt Independently of Hypertension
Autor/es:
RUKAVINA MIKUSIC NL; ROSON MI; DELLA PENNA SL; GORZALCZANY S; ZOTTA E; CHOI MR; TOBLLI JE; FERNÁNDEZ BE
Revista:
Antiinflamm Antiallergy Agents Med Chem
Editorial:
Bentham Science Publishers
Referencias:
Lugar: Sharjah; Año: 2016 vol. 15 p. 1 - 8
ISSN:
1871-5230
Resumen:
Background: Endothelial nitric oxide synthase (eNOS) is known tobe expressed in endothelium and smooth muscle cells of arteries. The aim ofthis study was to investigate the expression of eNOS in intimal and mediallayer of aorta from rats fed a high salt diet and its modulation by losartanand tempol.Methods: Rats were fed during three weeks with: normal salt diet (NS, 0.4%NaCl); high salt diet (HS, 8% NaCl); NS plus tempol 1 mM (NS-T); HS plustempol (HS-T); NS plus losartan 40mg.kg-1 (NS-L) and HS plus losartan(HS-L). Systolic blood pressure was recorded by the tail cuff method. Ratswere then anaesthetized and the thoracic aorta and small arteries (bronchialbranches of aorta) were processed to evaluate the expression of eNOS andaquaporin-1 (AQP-1) by immunohistochemistry.Results: HS group showed increased systolic blood pressure, increased eNOSand AQP-1 immunoexpression in the aorta intimal layer, and decreased eNOSimmunoexpression in the aorta medial layer, respect to NS group. Losartanand tempol prevented hypertension and changes in the expression of eNOS andAQP-1 of the intimal layer. However, only tempol increased the expression ofeNOS elicited by sodium overload in the medial layer of the aorta and smallarteries respect to HS group.Conclusions: A high salt diet decreases eNOS expression in vascular smoothmuscle layers of aorta and small arteries, which is reversed by tempol.These results suggest an adverse effect of oxidative stress on vascular eNOSin rats fed a high salt diet independently of hypertension.