Altered neutrophil activation in absence of IL-10 in a murine model of hemolytic uremic syndrome.

GONZALO EZEQUIEL, PINEDA; REARTE, BARBARA; CORDOBA MORENO, MARLINA; BRUBALLA, ANDREA; FERNANDEZ-BRANDO, ROMINA JIMENA; MEISS, ROBERTO; ISTURIZ, MARTIN; ALBA SOTO, CATALINA D; PALERMO, MARINA; RAMOS, MARIA VICTORIA

Mar del Plata

Congreso; Reunión Conjunta 2016 entre la Sociedad Argentina de Investigación Clínica (SAIC), la Sociedad Argentina de Inmunología (SAI) y la Sociedad Argentina de Farmacología Experimental (SAFE),; 2016

Sociedad Argentina de Investigación Clínica (SAIC), la Sociedad Argentina de Inmunología (SAI) y la Sociedad Argentina de Farmacología Experimental (SAFE),

Altered neutrophil activation in absence of IL-10 in a murine model of hemolytic uremic syndrome.Gonzalo Ezequiel Pineda1, Barbara Rearte2, Marlina Córdoba Moreno2, Andrea Bruballa1, Romina Fernandez-Brando1, Roberto Meiss3, Martin Isturiz2, Catalina Alba-Soto4,5, Marina Palermo1, Maria Victoria Ramos1.1Laboratorio de Patogenesis e Inmunologia de Procesos Infecciosos, IMEX-Academia Nacional de Medicina.2Laboratorio de Fisiologia de los procesos Inflamatorios, IMEX-Academia Nacional de Medicina.3Departamento de Patologia, Instituto de Estudios Oncologicos, Academia Nacional de Medicina4Instituto de Investigaciones en Microbiología y Parasitología Médicas (IMPaM; UBA-CONICET5 Departamento de Microbiología, Parasitología e Inmunología. Facultad de Medicina. Universidad de Buenos Aires.Hemolytic Uremic Syndrome (HUS), is a systemic disease caused by circulating Shiga-toxin (Stx) which mainly damages kidney. Besides Stx2, inflammatory response mediated by neutrophils (PMN) is essential for HUS evolution. We have demostrated that mice lacking IL-10 (IL-10-/-) had higher survival rate to Stx2 challenge than controls. The aim of this work was to determine the role of PMN in HUS evolution in absence of IL-10. IL-10-/- and WT mice were euthanized before and after of 1LD100 of Stx2 e.v. (24, 48 and 72h). Total leukocytes were counted and PMN identified as Ly6G+CD11b+ cells in blood and bone marrow. PMN production of reactive oxygen species (ROS) was evaluated without stimulus (basal) or after PMA stimulation, with DHR by FACS. In basal conditions, both groups showed similar absolute number of circulating PMN, although the percentage of PMN in bone marrow was increased in IL-10-/- mice (%PMN:IL-10-/-= 52.8±4.1,control=39.9±1.9*,*p