INVESTIGADORES
ALBA SOTO Catalina Dirney
congresos y reuniones científicas
Título:
Dendritic cells revert in an animal model of resorption rate: Tolerization
Autor/es:
SANDRA MARIA BLOIS, CATALINA ALBA SOTO, EDUARDO CHULUYAN, TERESA GENTILE, PETRA ARCK, RICARDO MARGNI.
Lugar:
Buenos Aires
Reunión:
Simposio; International symposium on dendritic cells and immunotherapy; 2002
Institución organizadora:
Maestria de Biologia Molecular Médica
Resumen:
The animal model of CDA/J female mice mated with DBA/2J male mice has increased fetal resoption rate. recently DCs have been described at the fetal- maternal interface in the human decidua. DCs localization preference to the basal layer of decidua and the intimate endometrial gland contact of DCs indicate that, theses glands may be subject to a form of active immune response. The aim of this study was to amalyse the effect of injecting the bone marrow derived DC (BM-DC) twice before pregnancy in the CDA/JxDBA/2J model. BM-DC were generated from the virgin female CBA/J mice (6 to 8 weeks old) by usinga modified version of Inaba´s et al. technique, some of the DCs were pulsed with paternal DBA/2J antigens lysate ex- vivo. Four different experimental groups were included:1) no treatment, 2)mice injected with conditioned medium, 3)immunized with DCs alone, 4) immunized with paternal DBA/2J antigens pulsed DCs, n=5. We confirmed the phenotype of our BM-DCs by flow cytometry before the adoptive transfer. CD11c is expressed by a substantial proportion of BM-DCs 70 to 90%. Theses cells also expressed MHC class ii and costimulatory molecules such as CD80 and CD86. Therapy with DCs alone as well as with antigens pulsed DCs significantly reduced the abortion rate from 23,8% in Group 1 to 17,6% in Group 2 and 2.2% in Group 3 and to 5% in Group 4. The animals treated with DC alone and those receiving antigen-pulsed DCs induced putative pathways of an immunological response necessary in the prevention of abortion.