IL-10 deficency protects renal damage in a murine model of hemolytic uremic syndrome.

GONZALO EZEQUIEL, PINEDA; FERNANDEZ-BRANDO, ROMINA JIMENA; REARTE, BARBARA; ISTURIZ, MARTIN; ALBA SOTO, CATALINA D; PALERMO, MARINA; RAMOS, MARIA VICTORIA

Ciudad Autónoma de Buenos Aires. SAP lisana

Congreso; IV LASID Meeting; LXIII Argentinean Immunology Society Meeting; II French-Argentinean Immunology Meeting; 2015

LASID, SAIC, FAIC.

IL-10 deficency protects renal damage in a murine model of hemolytic uremic syndrome.Gonzalo Ezequiel Pineda1, Romina Jimena Fernandez-Brando1, Barbara Rearte2, Martin Isturiz2, Catalina Alba-Soto3, Marina Palermo1, Maria Victoria Ramos1.1Laboratorio de Patogenesis e Inmunologia de Procesos Infecciosos, IMEX-Academia Nacional de Medicina.2Laboratorio de Fisiologia de los procesos Inflamatorios, IMEX-Academia Nacional de Medicina.3Instituto de Investigaciones en Microbiología y Parasitología Médicas (IMPaM). Facultad de Medicina. Universidad de Buenos Aires.Background: Hemolytic Uremic Syndrome (HUS), a vascular disease characterized by nephropathy, is caused by enterohemorraghic-producing-Shiga-toxins (Stx) bacteria. The aim of the work was to determine the role of IL-10 in HUS evolution.Methods: Mice lacking IL-10 (IL-10-/-) and control mice were injected i.v. with 1LD100 Stx2. Before and after 2, 48 and 72h Stx2, mice were bled to perform polymorphonuclear leukocytes (PMN) counts, urea and cytokine evaluation.Results: IL-10-/- mice had a higher survival rate to Stx2 than controls (%survivors:44 and 0, median survival: 120h and 96h, respectively, *p