INVESTIGADORES
ARANA Eloisa Irene
congresos y reuniones científicas
Título:
Human Memory B cells isolated from blood and tonsils are functionally distinctive
Autor/es:
ARANA E. I; BILLORDO, ARIEL; BAZ, PLACIDA; FAINBOIM, LEONARDO
Reunión:
Congreso; British Society for Immunology; 2014
Institución organizadora:
BSI
Resumen:
Human B cell studies in vitro have routinely used B lymphocytes purified from spleen, blood or tonsils irrespective of potential differences in their immunological traits. In this study, we compared the functional responses of total (CD19+) and memory B cells (CD19+/CD27+) isolated from blood and tonsils to different stimuli. Peripheral B cells showed enhanced survival and proliferation compared with their tonsillar equivalents when stimulated for 10 days. Stimulated B cells from both tissues secreted significantly greater amounts of cytokines (IL-6, IL-8, and GM-CSF) than unstimulated controls demonstrating their functional responsiveness. Analysis of CD27 expression over time indicated that the conditions that promoted survival and proliferation of peripheral memory B cells (Bmem), caused massive tonsillar Bmem death. Sorted tonsillar Bmem failed to expand but rapidly differentiated in antibody secreting cells (ASC) and subsequently underwent apoptosis. In contrast, circulating Bmem showed delayed activation and differentiation, but exhibited a longer lifespan and active proliferation. In addition, short-term stimulation of tonsillar Bmem resulted in the production of more IgG than their peripheral counterparts. At later time points, however, IgG production from the different B cells was reversed. Our findings imply that results generated either from tissue located or peripheral Bmem, may not always be applicable to the biological activities of other Bmem. Furthermore, they serve as starting point in understanding Bmem location coupled to a specialized role.