CARDILLO Alejandra Beatriz
congresos y reuniones científicas
Brugmansia candida Hairy Root Cultures in Bioreactors as an Anisodamine Production System
CARDILLO, ALEJANDRA BEATRIZ; OTALVARO, ANGELA MARIA; BUSTO, VICTOR; RODRIGUEZ TALOU, JULIAN; VELASQUEZ, MARIO ENRIQUE; GIULIETTI, ANA MARIA
Congreso; IBS 2010, 14th International Biotechnology Symposium and Exhibition; 2010
Brugmansia candida is a South American native plant that produces tropane alkaloids, such as hyoscyamine, anisodamine (6b-hydroxyhyoscyamine) and scopolamine.These alkaloids were traditionally used in medicine because of their anticholinergic activity. Recently there were described further therapeutical properties for anisodamine renewing the interest in these alkaloids. Nowadays, these alkaloids are produced by the exploitation of producer plants due to the cost and complexity that chemical synthesis represents. The scaling up of hairy root cultures is an interesting strategy for the pharmaceutical production of these compounds instead of the isolation from plants what could be affected by external factors such as temperature or pathogens. The aim of this work was to study the anisodamine and scopolamine production by B. candida hairy roots immobilized in a plastic mesh submerged into a 1.5 l stirred tank as an alternative production system. A volume of 1.2 L of Gamborg B5/2 culture medium was inoculated with 10 g FW of 20-days-old hairy roots. The culture was aerated by a stainless steel sparger located below the turbine by an airflow rate of 0.5 vvm. The alkaloids were analyzed and quantified by HPLC with UV detection. It was found that bioreactor cultures produced an increased biomass and alkaloids concentration compared to the processes carried out in Erlenmeyer flasks. The maximum concentration of anisodamine was 10.05 ± 0.76 mg/g DW being the predominant alkaloid at the harvest day (20 d). The results obtained are promissory for the development of an anisodamine production system.