CARDILLO Alejandra Beatriz
congresos y reuniones científicas
Anthraquinone production in M. citrifolia suspension cultures treated with proline analogs
PERASSOLO, MARIA; QUEVEDO, CARLA; MARTINEZ, CAROLINA; CARDILLO, ALEJANDRA BEATRIZ; BUSTO, VICTOR; GIULIETTI, ANA MARIA; RODRIGUEZ TALOU, JULIAN
Villa Carlos Paz
Congreso; SAIB 2008; 2008
Anthraquinones (AQs) are secondary metabolites produced by the Rubiaceae family that exhibit biological interesting properties. Different routes are involved in AQs synthesis: chorismic acid, produced by shikimate pathway (SP), is converted into isochorismic acid (IC) by the enzyme isochorismate synthase. IC reacts with á-ketoglutaric acid to form o-succinylbenzoic acid, the precursor of two rings of the AQs structure. The other ring is derived from an isoprene unit produced by the 2-C-methyl-D-erythritol-4-phosphate pathway. It was proposed that the proline cycle could be linked to the pentose phosphate pathway (PPP) by generating two NADP+ which are cofactors of the two first enzymes in the PPP. PPP supplies the SP with erithrose-4-phosphate. The aim of this work was to study a possible link between proline cycle and AQs production. Morinda citrifolia cell suspension cultures were treated with two proline analogs: azetidine-2-carboxylic acid (A2C; 25 and 50 µM) and thiazolidine-4-carboxylic acid (T4C; 100 and 200 µM). AQs content was higher (p<0.05) than the control line after 6 days of culture in all treated cells except from A2C 25 µM treatment, whereas total phenolics (TP) production was increased only with T4C 200 µM. After a 10 day-culture, both T4C treatments increased AQs and TP accumulation compared to control. These findings could be correlated with PPP stimulation.