A high expression of TLR4 supports the involvement of lipopolysaccharides (LPS) in nonalcoholic fatty liver disease (NAFLD).

INZAUGARAT MARIA EUGENIA; GARCÍA CECILIA C; BAZ PLÁCIDA; CHERÑAVSKY ALEJANDRA C

Londres

Encuentro; 49th Annual Meeting of the European Association for the Study of the Liver; 2014

EASL

Toll-like receptor (TLR) 4, involved in lipopolysaccharides (LPS) recognition, is expressed by subsets of memory activated T cells. Increased LPS levels in non alcoholic fatty liver diseases (NAFLD) might be involved in its immunpathogenesis. We aimed to evaluate TLR4 expression in circulating and intrahepatic central/ effector memory lymphocytes. Also to characterize intrahepatic T cell differentiation in NAFLD. Patients and Methods: Peripheral blood mononuclear cells (PBMC) and liver biopsies were obtained [25 adult patients (NAFLD); 20 healthy controls (Co)]. Total or CD45RO+ PBMC and liver cell suspensions were stained (anti-CD4/-CD8)/-CCR7/-TLR4 mAbs) and analyzed by flow cytometry. A CCR7 index (ICCR7 CD4+ or CD8+ = % CCR7+/ CCR7-) was calculated. IFN-, CCL20 and T-bet intrahepatic expression was evaluated by real-time PCR, 2-ΔΔCt method and Mann-Whitney test. Results: ICCR7 CD4+ and CD8+ are decreased in total PBMC (p=0.007 and 0.004) and CD45RO+ PBMC (p