PERSONAL DE APOYO
BAZ Placida
artículos
Título:
Flow Cytometry TUNEL Standardization for Assaying Sperm DNA Fragmentation
Autor/es:
SUSANA CURI; PATRICIA CHENLO; LUIS A. BILLORDO; PLÁCIDA BAZ; MELBA SARDI; MELBA SARDI; HERBERTO REPETTO; GABRIELA R.MENDELUK. ; MERCEDES N. PUGLIESE
Revista:
JOURNAL OF ANDROLOGY
Editorial:
AMER SOC ANDROLOGY, INC
Referencias:
Año: 2012 vol. 33 p. 1 - 1
ISSN:
0196-3635
Resumen:
We read with great interest Muratori?s paper (Muratori
et al, 2010), because we believe that terminal deoxynucleotidyl
transferase?mediated dUTP nick end labeling
(TUNEL) assay standardization is a matter of great
concern and that there are certain steps that must be
followed to achieve accurate results.
We agree with the authors of the paper but feel the
need to stress several important considerations. As the
authors mention in their introduction, the labeling of
DNA breaks should be carried out by nuclear staining
with propidium iodide (PI; Muratori et al, 2008). We
have noted in oligozoospermia samples a clear increase
in the percentage of PI-negative events with forward
scatter/side scatter similar to sperm, yielding lower
results. The upper sperm count limit that could be
considered a threshold for this phenomenon is difficult
to establish; thus, TUNEL/PI should be applied to all
samples, and reference values should be recalculated for
future clinical use.