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Título:
A differential distribution of CD4/CD8 subpopulations and IFN-£^ƒnproduction in child and adults with Nonalcoholic steatohepatitis (NASH)
Autor/es:
INZAUGARAT MARIA EUGENIA; FERREYRA SOLARI NE; BAZ PLÁCIDA; CHERÑAVSKY ALEJANDRA C
Lugar:
Buenos Aires
Reunión:
Congreso; First French-Argentine FAIC Immunology Congress; 2010
Institución organizadora:
SAI-SAP-GRCF-SFI
Resumen:
Obesity and metabolic syndrome (MS) are risk factors for nonalcoholic steatohepatitis (NASH). Lymphocyte recruitment into adipose and hepatic tissues exacerbates the inflammation. Objective: To examine the distribution of CD4/CD8 subpopulations and to measure its IFNγ production. Pediatric (12 Ped, age < 18y) and adults (12 Ad, age > 18y) with NASH (N), obeses (O) with MS without hepatic damage (15 OPed, 6 OAd) and 30 healthy Ped / Ad controls were studied. Peripheral blood mononuclear cells (PBMC) obtained by Ficoll-Hypaque were co stained with anti-CD4 or -CD8 PerCP and anti-CD45RO or -CD45RA FITC and analyzed by flow cytometry. For intracellular detection, PBMC were stimulated with PMA/Ionomycin/brefeldin and stained with anti-CD4 or -CD8/-IFNγ mAbs. Kruskal-Wallis and Mann-Whitney tests were performed. Results: In Ped NASH, total CD4+ subpopulation is decreased(pc= 0.039 and 0.012, vs. Co and OPed). Lack of differences for CD8+ subpopulation notwithstanding, naïve (pc