Reactive Species of Oxygen production by Monocytes and Neutrophils in pediatric patients with Nonalcoholic Steatohepatitis

INZAUGARAT MARIA EUGENIA; CHERÑAVSKY ALEJANDRA C; BAZ PLÁCIDA; FERREYRA SOLARI NE

Buenos Aires

Congreso; First French- Argentine Immunology Congress; 2010; 2010

SAI-SAP-GRCF-SFI

Nowadays, obesity as a component of the metabolic syndrome (MS) is considered a major worldwide pandemic associated with several metabolic conditions, including nonalcoholic steatohepatitis (NASH). Reactive species of oxygen (ROS) produced by monocytes (Mo) and Neutrophils (Ne), infiltrating the adipose tissue (AT) and the liver, is associated with inflammation and tissue damage. Objective: to evaluate on the effect of fatty acids on oxidative burst in monocytes and neutrophils Methods: 10 patients with NASH (N), 20 obese individuals with MS without hepatic damage (Ob) and 20 healthy controls (Co) from our pediatric population (age < 18 y) were included. Mononuclear cells were obtained from heparinised peripheral blood (PBMC) by Ficoll-Hypaque gradient. Lysis of whole blood was performed for assays with Ne. PBMC and Ne were independently incubated with dichlorofluorescein-diacetate [5 µM], stimulated with Phorbol-Myristate Acetate (PMA, [100 ng/ml]), Palmitic acid [500 µM] and Linoleic acid [200 µM] and analyzed by Flow Cytometry. A Stimulation Index (SI) was defined as: mean fluorescence intensity (MFI) stimulated cells/MFI-unstimulated cells. Statistical analysis was performed by Kruskal-Wallis and Mann-Whitney with Bonferroni correction. Results: A higher SI was found for Ne from Ob (pc=0.006) and patients with NASH (pc=0.006) compared to Co. Stimulation of Mo with PMA induced higher production of ROS in Op related to Co (pc=0.002) and NASH (pc