An authomatic wrapped phase gradient - based method for spherical aberration correction in Digital Holographic Microscopy

A. C. MONALDI; G. G. ROMERO; A. V. BLANC; C. M. CABRERA

OPA Journal

sedoptica

Año: 2018 vol. 51 p. 490321 - 490328

0030-3917

Most Digital Holographic Microscopy architectures introduce a Microscope Objective in the object arm of the micro-interferometer in order to increase lateral resolution but, as a consequence, a spherical phase aberration arises. The phase distortion must be corrected to achieve reliable phase information linked to the microscopic object under study. In this work, we present a fast and simple numerical method for automatically compensate the phase curvature from the wrapped phase gradient of the reconstructed hologram. Since non-unwrapping methods are required, computational cost is significantly reduced. Furthermore, no additional holograms recording are needed and it does not required prior knowledge of the object or the setup. The method is experimentally demonstrated by a phase contrast imaging of a Pediastrum cell.