TLR4 and TLR9 expression might modulate the adaptive immune response in experimental nonalcoholic steatohepatitis

ALEGRE NS; INZAUGARAT ME; GARCÍA CC; BILLORDO ARIEL; WALD M; A. C. CHERÑAVSKY

Buenos Aires

Congreso; FAIC 2015; 2015

SFI/SAI/LASID

Toll like receptors (TLRs) T cell expression allows the modulation of adaptive immune response. We have demonstrated that Curcumin (Cur) oral treatment modulates intrahepatic immunological activity in high fat diet (HFD)-induced model of nonalcoholic steatohepatitis (NASH). We aimed to evaluate TLR4 and TLR9 expression in liver and spleen T cells, the association between TLRs and tissue injury and the in vivo Cur effects in HFD-fed mice. Methods C57BL/6 mice were fed normal chow (NC) or high fat diet (HFD) (10/group). After 4 weeks of feeding, Cur (2g/kg diet) was co-administered to half of each group (NC+Cur, HFD+Cur). Mice were sacrificed then spleen and liver were weighed. Cell suspensions were obtained and staining with specific CD4-PE/APC, CD8-APC, TLR4-PE and TLR9-FITC for flow cytometry analysis. Liver histological activity score (NAS) was also performed. %TLR4+ cells and TLR9 mean fluorescence intensity (MFI) were analyzed by two-way ANOVA with Bonferroni`s post-test. Spearman's test was used for correlation. Results HFD increased spleen (TLR4+CD8+ p