PERSONAL DE APOYO
BILLORDO Luis Ariel
artículos
Título:
Alterations in innate and adaptive immune leukocytes are involved in paediatric obesity
Autor/es:
M. E. INZAUGARAT; L. A. BILLORDO; F. VODÁNOVICH; G. M. CERVINI; P. L. CASAVALLE; C. VEDIRE; A. C. CHERÑAVSKY
Revista:
Pediatric obesity
Editorial:
Wiley & Blackwell
Referencias:
Año: 2013 p. 1 - 10
ISSN:
2047-6310
Resumen:
SummaryBackground: Adipose tissue is the main source of the cytokines and adipokines that are increased in thecontext of obesity. The production of reactive oxygen species (ROS) and cytokines by circulating immunecells can be regulated by these pro-inflammatory factors even before infiltration into adipose tissue.Objective: To investigate the alterations that can occur in circulating monocytes and lymphocytes inpaediatric obesity.Methods: In this study, 54 paediatric obese patients and 30 age-matched metabolically healthy individualswere enrolled. Intracellular cytokines were analyzed after phorbol myristate acetate (PMA) or leptin plusPMA stimulation of lymphocytes and monocytes by flow cytometry. ROS generation was measured usingdichlorofluorescein-diacetate. Both a ?stimulation index? and a ?fold of increase? were calculated for statisticalpurposes.Results: Both interferon gamma (IFN-g) production by circulating CD4+ and CD8+ lymphocytes and ROSproduction by monocytes following PMA stimulation were increased in obese patients. Leptin induced anincreased production of IFN-g in both subsets of T cells and tumour necrosis factor alpha in monocytes, andlinoleic acid induced a higher ROS production in monocytes.Conclusions: The distinct functional responses of circulating cells suggest that alterations in both innateand adaptive immune cells are involved in the maintenance of low-grade inflammation in paediatric obesity