PERSONAL DE APOYO
PICCO Maria Elisa
artículos
Título:
Protein kinase D1 inhibition interferes with mitosis progression
Autor/es:
MARTÍNEZ-LEÓN, EDUARDO; AMABLE, GASTÓN; JÁCAMO, RODRIGO; PICCO, MARÍA ELISA; ANAYA, LAURA; ROZENGURT, ENRIQUE; REY, OSVALDO
Revista:
JOURNAL OF CELLULAR PHYSIOLOGY
Editorial:
WILEY-LISS, DIV JOHN WILEY & SONS INC
Referencias:
Año: 2019 vol. 234 p. 20510 - 20519
ISSN:
0021-9541
Resumen:
Protein kinase D1 (PKD1) plays a vital role in signal transduction, cell proliferation, membrane trafficking, and cancer; however, the majority of the studies up to date had centered primarily on PKD1 functions in interphase, very little is known about its role during cell division. We previously demonstrated that during mitosis PKD1 is activated and associated with centrosomes, spindles, and midbodies. However, these observations did not address whether PKD1 was associated with mitosis regulation. Accordingly, we used rapidly acting PKD-specific inhibitors to examine the contribution of PKD1 the sequence of events in mitosis. We found that although PKD1 overexpression did not affect mitosis progression, suppression of its catalytic activity by two structurally unrelated inhibitors (kb NB 142-70 and CRT 0066101) induced a significant delay in metaphase to anaphase transition time. PKD1 inhibition during mitosis also produced the appearance of abnormal spindles, defects in chromosome alignment, and segregation as well as apoptosis. Thus, these observations indicate that PKD1 activity is associated with mitosis regulation.