INVESTIGADORES
FENOY Ignacio Martin
congresos y reuniones científicas
Título:
DUAL ROLE OF THE TLR-AGONIST PROFILIN PROTEIN FROM TOXOPLASMA GONDII: STUDY OF THE ADJUVANT AND IMMUNOGENIC VALUE IN A VACCINE FORMULATION IN TWO MOUSE STRAINS WITH DIFFERENT SUSCEPTIBILITY
Autor/es:
ARCÓN, N; M PICCHIO; V SANCHEZ; R MORETTA; I FENOY; A SOTO; M PERRONE SIBILIA; ALDIRICO MA; A GOLDMAN; V MARTÍN
Lugar:
Mar del Plata
Reunión:
Congreso; LXVI Reunión Anual de la Sociedad Argentina de Inmunología 2018; 2018
Resumen:
Toxoplasmosis is a disease that affects 30% ofthe world?s popu­lation. At present, there are no pharmacological treatmentsthat eliminate the parasite or vaccines that confer protection to the host. Theaim of the present work was to study the immunogenicity of a vaccineformulation containing a recombinant form of the T. gon­dii Profilinprotein (rTgPF), a TLR ligand, in combination with the dense granule GRA7protein (rGRA7), in two mouse strains with dif­ferent susceptibility to chronictoxoplasmosis. BALB/c and C57BL/6 mice were intradermally immunized 3-timeswith a 2-week interval with: rGRA7, rTgPF, rGRA7+rTgPF, rGRA7+ACF. Naive micewere used as control. While in BALB/c mice rGRA7+rTgPF vaccination generated ananti-GRA7 humoral response with a Th1 profile and rGRA7+ACF showed a mixedprofile, both formulations induced a mixed profile in C57BL/6 mice. Ex vivo stimulationof splenocytes with rGRA7 induced significant proliferative responses(p