INVESTIGADORES
FENOY Ignacio Martin
congresos y reuniones científicas
Título:
DUAL ROLE OF THE TLR-AGONIST PROFILIN PROTEIN FROM TOXOPLASMA GONDII: STUDY OF THE ADJUVANT AND IMMUNOGENIC VALUE IN A VACCINE FORMULATION IN TWO MOUSE STRAINS WITH DIFFERENT SUSCEPTIBILITY
Autor/es:
ARCÓN, N; M PICCHIO; V SANCHEZ; R MORETTA; I FENOY; A SOTO; M PERRONE SIBILIA; ALDIRICO MA; A GOLDMAN; V MARTÍN
Lugar:
Mar del Plata
Reunión:
Congreso; LXVI Reunión Anual de la Sociedad Argentina de Inmunología 2018; 2018
Resumen:
Toxoplasmosis is a disease that affects 30% ofthe world?s population. At present, there are no pharmacological treatmentsthat eliminate the parasite or vaccines that confer protection to the host. Theaim of the present work was to study the immunogenicity of a vaccineformulation containing a recombinant form of the T. gondii Profilinprotein (rTgPF), a TLR ligand, in combination with the dense granule GRA7protein (rGRA7), in two mouse strains with different susceptibility to chronictoxoplasmosis. BALB/c and C57BL/6 mice were intradermally immunized 3-timeswith a 2-week interval with: rGRA7, rTgPF, rGRA7+rTgPF, rGRA7+ACF. Naive micewere used as control. While in BALB/c mice rGRA7+rTgPF vaccination generated ananti-GRA7 humoral response with a Th1 profile and rGRA7+ACF showed a mixedprofile, both formulations induced a mixed profile in C57BL/6 mice. Ex vivo stimulationof splenocytes with rGRA7 induced significant proliferative responses(p