Immune-modulatory effects of the serine protease inhibitor-1 of Toxoplasma gondii on innate immune response

VANESA SÁNCHEZ, MATÍAS PERRONE SIBILIAC, MARIANO PICCHIO, NADIA ARCON, ARIADNA SOTTO, MARÍA DE LOS ANGELES ALDIRICO, IGNACIO FENOY, ALEJANDRA GOLDMAN, VALENTINA MARTIN

Simposio; Reunión Conjunta sociedades biociencias; 2017

Toxoplasma gondii infects approximately one-third of the world population, making it oneof the most successful parasitic organisms. T. gondii serine protease inhibitor-1 (TgPI-1) is a potentinhibitor of neutrophil elastase, trypsin and chymotrypsin but its role during natural infection isnot already known. It has been reported that other serine protease inhibitors are able to modulatethe immune system. Previously we showed that TgPI-1 was able to down-modulate both a Th2 andTh1 type immune responses already established. Those results led us to explore the ability of TgPI-1 to modulate the innate immune response. In this work we used the J774-A1 murine macrophagecell line to study this possible modulatory role. Cells were stimulated with lipopolysaccharide (LPS)or exposed to residual oil fly ash (ROFA), in the presence of a recombinant form of TgPI-1 (rTgPI-1).We observed that rTgPI-1 was able to decrease nitric oxide production induced by LPS stimulationwhen compared to LPS alone (p