INVESTIGADORES
FENOY Ignacio Martin
congresos y reuniones científicas
Título:
The TLR agonist profilin protein from Toxoplasma gondii generates protection in an experimental vaccine
Autor/es:
NADIA ARCON, MARIANO S. PICCHIO, IGNACIO M. FENOY, MATÍAS D. PERRONE SIBILIA, ARIADNA S. SOTO, VANESA R. SÁNCHEZ, MARÍA DE LOS ÁNGELES ALDIRICO, ALEJANDRA GOLDMAN, VALENTINA MARTIN.
Reunión:
Simposio; Reunión Conjunta sociedades biociencias; 2017
Resumen:
Toxoplasmosis is a disease that affects 30% of the world´s population.At present, there are no pharmacological treatments that eliminate the parasiteor vaccines that confer protection to the host. In this work we studied the adjuvantand/or immunogenic value of a recombinant form of T. gondii Profilin protein (rTgPF), a TLR ligand, in a vaccinestrategy using a murine model of chronic toxoplasmosis. BALB/c mice, with highresistance to this infection, were intradermal immunized 3 times with a 2-weekinterval with: rGRA7 (recombinant T.gondii dense granule 7 protein), rTgPF, rGRA7+rTgPF or rGRA7+ACF.The anti-GRA7humoral response showed a Th1 profile when rTgPF was used as adjuvant(IgG1/IgG2a ratio: 0,6±0,5), while rGRA7+ACF showed a mixed profile (IgG1/IgG2a ratio: 1,3±0,5). Splenocytes from rGRA7+rTgPF and rGRA7+ACF mice stimulatedwith rGRA7, showed similar levels of proliferative responses that weresignificantly higher than the control group (p