INVESTIGADORES
FENOY Ignacio Martin
artículos
Título:
Homologous Prime-boost strategy with TgPI-1 improves the immune response and protects highly susceptible mice against chronic Toxoplasma gondii infection
Autor/es:
SANCHEZ VANESA; FENOY IGNACIO; PICCHIO MARIANO; SOTO ARIADNA; ARCÓN NADIA; GOLDMAN ALEJANDRA; MARTIN VALENTINA
Revista:
ACTA TROPICA
Editorial:
ELSEVIER SCIENCE BV
Referencias:
Lugar: Amsterdam; Año: 2015
ISSN:
0001-706X
Resumen:
Although subunit-based vaccines are safer than live or attenuated pathogen vaccines, they are generally weak immunogens. Thus, proper combination of immunization strategies and adjuvants are needed to increase their efficacy. We have previously introduced the serine protease inhibitor-1 of T. gondii (TgPI-1) as a new antigen with promissory potential to be included in a multiantigenic vaccine against toxoplasmosis. Since TgPI-1 is capable to inhibit a broad range of proteases including neutrophil elastase, we hypothesized that it could be an important therapeutic target. Here, we report a homologous prime-boost vaccination strategy that combines administration of TgPI-1 by intradermal and intranasal routes. We achieved an improvement in the protection against infection in the highly susceptible C57BL/6 mouse strain, in comparison with protocols using a single vaccination route. This enhanced protection was related to the generation of both mucosal and systemic immune responses. In addition, adoptive transference of mesenteric lymph node cells from vaccinated to naïve mice induced significant protection against infection. In conclusion, TgPI-1 prime-boost vaccination strategy improves systemic and mucosal immunity against T. gondii infection in C57BL/6 mice, highlighting the importance of testing different delivery systems in the case of a particular antigen.