ROMERO Fernando Matias
congresos y reuniones científicas
Secretion of putrescine by Pseudomonas syringae functions as an antioxidant defense during plant invasion
SOLMI L; ROSSI FR; ROMERO FM; RUIZ OA; GÁRRIZ A
Congreso; XXXII Reunión Argentina de Fisiología Vegetal (RAFV) y XVI Congreso Latinoamericano de Fisiología Vegetal; 2018
Polyamines such as putrescine, spermidine and spermine are important for virulence in phytopathogens. In this work, we were prompted to explore whether the secretion of this polyamine by P. syringae functions to cope with oxidative stress. Our analysis demonstrated that bacterial growth is affected by sub lethal concentrations of H2O2. In addition, P. syringae induced the secretion of putrescine in response to this oxidative environment, leading to the increase in its concentration at the extracellular space. Disruption of polyamine biosynthetic genes led to an increase of intracellular reactive oxygen species, demonstrating that these compounds are involved in maintaining redox homeostasis. Nevertheless, the addition of putrescine to culture media was not able to ameliorate the effects of oxidative stress neither restore bacterial growth in the presence of H2O2. These results shed more light to the roles played by polyamines during bacterial pathogenesis.