ROMERO Fernando Matias
congresos y reuniones científicas
Antimicrobial activity of a Kazal-type inhibitor from Toxoplasma gondii against plant pathogens
SÁNCHEZ LÓPEZ EF; CORIGLIANO MG; PARIANI SA; ROMERO FM; PIECKENSTAIN FL; CLEMENTE M
Mar del Plata
Congreso; 51 Annual Meeting Argentine Society for Biochemistry and Molecular Biology; 2015
Plants are susceptible to be infected by several pathogens that secrete proteinases. A serine proteinase inhibitor of Toxoplasma gondii (TgPI) has shown to be a potent trypsin, chymotrypsin and elastase inhibitor. Since serine inhibitors have been established as an effective protective system for plants, we propose to evaluate the antimicrobial activity of the recombinant TgPI (rTgPI) against plant pathogens with the object to use this inhibitor as a novel strategy for improving plant pathogen resistance. Previously, we showed that rTgPI has bacteriostatic effect on Pseudomonas growth. Following with the characterization of the inhibitory properties of rTgPI, the germination of Botrytis cinerea conidia in culture medium supplemented with rTgPI was analyzed. Germination rates of B. cinerea conidia in the medium containing rTgPI were significantly lower than control. To investigate whether rTgPI is able to inhibit the infection in planta, A. thaliana were coinoculated with B. cinerea or P. siryngae and rTgPI. After B. cinerea infection, necrotic area was measured. Leaves co-inoculated showed a reduction of 70-80% of necrotic area respect to the controls. Similarly, bacterial counts were significantly reduced in leaves co-inoculated with P. siryngae and rTgPI respect to the controls. In conclusion, rTgPI would be able to inhibit the infectivity capacity of these plant pathogens.