ROMERO Fernando Matias
congresos y reuniones científicas
Role of botrydial and botcinins in the interaction between Botrytis cinerea and competing fungi
VIGNATTI P; GONZALEZ ME; ROMERO FM; GONZÁLEZ COLLADO I; VIAUD M; PIECKENSTAIN FL
Mar del Plata
Congreso; 51 Annual Meeting Argentine Society for Biochemistry and Molecular Biology; 2015
Aim: To analyze the fungi toxic potential of botrydial and botcinins produced by B. cinérea and to determine if these compounds play a role in the antagonism between this phytopathogenic fungus and competingmicroorganisms.Methodology: Dual culture assays were used to screen a collection of microorganisms isolated from natural environments for the presence of fungi inhibited by wild-type (WT) B. cinerea. These fungi were designated as FIBBC. B. cinerea mutants unable to produce botrydial and botcinins were also analyzed for their ability to inhibit the above mentioned fungi. Botrydial and botcinins were purified from WT B. cinérea cultures and their inhibitory activityon conidia germination and germ-tube growth of FIBBC was analysed.Results: Fungal strains of the genus Trichoderma inhibited in dual cultures by WT B. cinerea were identified. B. cinerea mutants that produce no botrydial and botcinins still antagonized the abovementioned FIBBC. Botrydial and botcinins inhibited conidia germination and germ tube growth of FIBBC.Conclusion: Botrydial and botcinins are toxic to potential fungal competitors of B. cinerea. However, the relevance of each of these compounds in the inhibition activity of B. cinerea towards these fungi is difficult to be estimated on the basis of mutant analysis, probably due to functional redundancy between the different toxins.