ICIAGRO-LITORAL   28228
INSTITUTO DE CIENCIAS AGROPECUARIAS DEL LITORAL
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
The Insecticidal-Protein Repertory of 14 Xenorhabdus Strains Isolated from Argentina
Autor/es:
CABALLERO, PRIMITIVO; DEL VALLE, ELEODORO; BERRY, COLIN; PALMA, LEOPOLDO; FRIZZO, LAUREANO S.
Reunión:
Conferencia; 1st International Electronic Conference on Toxins; 2021
Institución organizadora:
Sciforum (Basel, Switzerland)
Resumen:
Entomopathogenic nematodes belonging to the genus Steinernema are able to infest and kill insect hosts in association with their resident, entomopathogenic symbiont bacteria in the gram-negative genus Xenorhabdus (Enterobacteriaceae). However, only a few species of Xenorhabdus have been isolated from their hosts and their insecticidal properties reported. Here we performed the genome sequence analysis of 14 Xenorhabdus strains isolated from Steinernema nematodes in Argen-tina, able to kill 6th instar Galleria mellonella (Lepidoptera: Pyralidae) larvae. The 14 draft genome sequences encoded a total of 110 putative insecticidal proteins (mostly Tc, Pra/Prb and Mcf homo-logs) plus other virulence factors with similarity to putative nematocidal proteins and chitinases. The genome sequences of strains Flor, 5, PSL, Reich, 42, Vera, M, 18, Cul, DI, 12, 38, 3 and ZM ex-hibited 4, 9, 2, 10, 9, 5, 7, 9, 10, 7, 3, 18, 8 and 8 putative insecticidal genes, respectively. Some strains carried their predicted insecticidal protein genes arranged into putative pathogenicity islands. Av-erage nucleotide identity (ANI) calculations were also performed and allowed the identification of three strains that should be considered as members of two novel Xenorhabdus genomospecies (strains PSL + Reich; and strain 12). In this work, we provide a dual insight into the diversity of the species belonging to the Xenorhabdus genus and into their predicted insecticidal protein repertory, which is currently under investigation.