Effects of Bacillus safensis S9 on soybean plants (Glycine max L. Merrill) under salinity

BATISTUTA, R.; CÉCCOLI, GABRIEL; DAURELIO, LUCAS D.; OLIVELLA, LAURA; BOUZO, CARLOS; BARENGO, PAMELA B.; GAIDO, J.; ESPARIZ, MARTIN

Santa Fe

Congreso; RAFV CONFERENCE 2021 ? XXXIII Argentinian meeting of Plant Physiology; 2021

Sociedad Argentina de Fisiología Vegetal

The increase in soils salinity causes important losses in crops yields. Plant growth-promoting bacteria (PGPB) are alternative treatments to alleviate this abiotic stress. In this work, we evaluate the effects of Bacillus safensis S9 on soybean plants grown under salinity. Nidera® seeds genotype were treated with S9 grown in Lysogeny Broth medium (LB), overnight (ON), diluted to optical density (OD) of 1 during 5 minutes. Control plants were treated with LB only. Plants were grown in 5 L pots with sand and irrigated with NaCl solution from stage V2 (only salt treated plants) in a greenhouse. Water Potential (WP, upper and lower canopy) and Water Use Efficiency (WUE) were measured in R2.5 stage and membrane stability (Ion Leakage) in R5 stage. Statistical analysis was performed using Infostat® Software. Salinity decreased WP in order of 60 % and increased WUE in 52.8%. Plants under 0 mM increased WUE in 132% by effect of bacterial treatment . An increase of cell membranes damage was observed in presence of 75 mM NaCl related to 0 mM in both bacterial treatment (92%). In conclusion, the treatment with S9 had a negative effect on WP both control and stressed plants. Bacterial treatment increased WUE in control plants, while there were no changes under salinity. No differences in cell membrane stability was observed in the 4 treatments. Bacillus safensis S9 inoculation did not help plants to recover from saline negative effects. New approaches will be necessary to propose Bacillus safensis S9 as an effective inoculant.