Antagonism of Bacillus safensis strain against phytopathogenic bacteria

TORRES MANNO, MARIANO A.; DAURELIO, LUCAS D.; GAIDO JIMENA; ESPARIZ, MARTÍN; OLIVELLA LAURA; PETITTI TOMÁS

Buenos Aires

Congreso; LVII SAIB Meeting - XVI SAMIGE Meeting; 2021

SAIB - SAMIGE

Citrus canker caused by Xanthomonas citri subsp. citri (Xcc), is a bacterial disease which affects all the citrics. One alternative to manage it is the use of antagonist bacteria. The aim of this work was to investigate the antagonist activity of Bacillus safensis (S9) against Xcc. The activity was tested by diffusion assays. Xcc and S9 were grown overnight in Luria Bertani (LB) and potato dextrose (PD) medium, respectively, with continuous agitation at 28°C and then, were diluted to a concentration of 108 CFU/mL. Petri dishes were covered with 15 mL of LB-agar containing 100 μL of the Xcc dilution. Once the medium was solidified, 4 μL drops of S9 were inoculated 3 times in each Petri dish, and the experiment was made by triplicate. After 48 hours of incubation at 28°C, the inhibition zone was measured, and the average inhibition area was calculated as IA = average area of the inhibition zone - average area of the colony. A significant inhibition area of 5.18 cm2 was obtained (one-sample t- test, p