INVESTIGADORES
DOMENE Sabina
artículos
Título:
Expression of acid-labile subunit (ALS) in developing and adult zebrafish and its role in dorso-ventral patterning during development
Autor/es:
LANDI, ESTEFANÍA; KARABATAS, LILIANA; SCAGLIA, PAULA; PISCIOTTANO, FRANCISCO; GUTIÉRREZ, MARIANA; RAMÍREZ, LAURA; BERGADÁ, IGNACIO; REY, RODOLFO A.; JASPER, HÉCTOR GUILLERMO; DOMENÉ, HORACIO MARIO; PLAZAS, PAOLA VIVIANA; DOMENÉ, SABINA
Revista:
GENERAL AND COMPARATIVE ENDOCRINOLOGY
Editorial:
ACADEMIC PRESS INC ELSEVIER SCIENCE
Referencias:
Año: 2020 vol. 299
ISSN:
0016-6480
Resumen:
Mammalian acid-labile subunit (ALS) is a serum protein that binds binary complexes between Insulin-likegrowth factors (IGFs) and Insulin-like growth factor-binding proteins (IGFBPs) extending their half-life andkeeping them in the vasculature. Human ALS deficiency (ACLSD), due to homozygous or compound heterozygous mutations in IGFALS, leads to moderate short stature with reduced levels of IGF-I and IGFBP-3. There isonly one corresponding zebrafish ortholog gene and it has not yet been studied. In this study we elucidate therole of igfals during zebrafish development. In zebrafish embryos igfals mRNA is expressed throughout development, mainly in the brain and subsequently also in the gut and swimbladder. To determine its role duringdevelopment, we knocked down igfals gene product using morpholinos (MOs). Igfals morphant embryos displayed dorsalization in different degrees of severity, including a shortened trunk and loss of tail. Furthermore,co-injection of human IGFALS (hIGFALS) mRNA was able to rescue the MO-induced phenotype. Finally, overexpression of either hIGFALS or zebrafish igfals (zigfals) mRNA leads to ventralization of embryos including areduced head and enlarged tail. These findings suggest that als plays an important role in dorso-ventral patterning during zebrafish development.