INVESTIGADORES
MINAHK Carlos Javier
congresos y reuniones científicas
Título:
The role of respiratory oxidase in the mechanism of action of microcin J25
Autor/es:
GALVAN AE; CHALON M; SCHURIG-BRICCIO L; MINAHK C; GENNIS R; BELLOMIO A
Lugar:
Córdoba
Reunión:
Congreso; LII Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; 2016
Resumen:
The antibacterialpeptide microcin J25 (MccJ25) displays an antibiotic activity againstSalmonella, Shigella and Escherichia coli. MccJ25 has two cellulartargets, the RNA polymerase and the respiratory chain. The terminaloxido-reductases inE. coli respiratory system are thecytochromesbo3andbd. We studied the effect of MccJ25 inE. coliC43cytochrome mutant strains. The oxygen consumption was diminished byMccJ25 in the wild type strain and in the mutant strain lacking thecytochromebo3, but did not have any effect in ∆bdI strain. In thesame way, superoxide production in isolated membranes was increasedmore than 100 % in the control and ∆bo3strain, whereas incytochromebdmutant such increment was not observed. Moreover, workingwith purified cytochromes, MccJ25 inhibited about 25 % the ubiquinoloxidase activity only on cytochrome bdI, while under identicalexperimental conditionsbo3oxidase was insensitive to the peptide.These results demonstrate that cytochrome bdI plays an important rolein the microcin J25 mechanism of action on the respiratory chain ofE.coli. Our findings would provide a new insight into the applicationof MccJ25 in food or pharmaceutical industries.p { margin-bottom: 0.25cm; line-height: 115%; }