INVESTIGADORES
MINAHK Carlos Javier
congresos y reuniones científicas
Título:
Stability of the point mutation of the Salmonella thyphimurium rcsc11 attenuated strain
Autor/es:
TORRES, MA; PESCARETTI, MM; MINAHK, C; DELGADO, MA
Lugar:
Rosario
Reunión:
Conferencia; L Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; 2014
Institución organizadora:
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Resumen:
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The foodborne
diseases are usually caused by Escherichia coli, Shigella, Listeria
and Salmonella, which is the most prevalent bacterium in
Argentina. The Salmonella infection involves many steps where the
modulation of genes located in different pathogenesis islands are
coordinately controlled. The RcsCDB system of Salmonella controls the
colanic acid, flagellar and biofilm synthesis, as well as
others virulence processes. Previously, we have studied the
rcsC11 mutant harboring a point mutation in the histidine kinase
domain of RcsC sensor. This change produces the RcsCDB system
constitutive activation, resulting in a mutant with mucoid
phenotype and attenuation of virulence. Here, we evaluated the
ability the rcsC11 mutant to infect non-phagocytic and phagocytes
eukaryotic cells. We also investigated the reversion degree of the
rcsC11 mutant to wild-type state by loss of the mucoid phenotype, by
means of temperature changes, crop aging and chemical
mutagenic agent challenges. We found that the rcsC11 mutant
produced strong immunogenic response and it was unable to replicate
in macrophages. Moreover, no revertant strains were isolated in any
of the treatment analyzed, strongly suggesting that the rcsC11
mutation would be fairly stable. The results of this study represent
a significant contribution that supports the use of the rcsC11 mutant
as candidate for vaccines development.