INVESTIGADORES
MINAHK Carlos Javier
congresos y reuniones científicas
Título:
Polyphenols-membrane interaction and protection against lipid oxidation. A biophysical study
Autor/es:
DE ATHAYDE MONCORVO, A; MINAHK, C; ACUÑA, L; DUPUY, F
Lugar:
Rosario
Reunión:
Congreso; L Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; 2014
Institución organizadora:
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Resumen:
p { margin-bottom: 0.25cm; line-height: 120%; } Polyphenols are a heterogeneous group of secondary metabolites, characterized by the presence of two or more phenolic groups. The characterization of the interaction of polyphenols with membranes may provide insights on the mechanism of action of the antioxidant and potentially protective activity of polyphenols on biological membranes. The aim of the present work was to determine the membrane interaction of resveratrol, naringenin, epigallocatechin gallate and enterodiol by means of biophysica l techniques in model systems. Also, the extent of lipid oxidation in the presence of polyphenols was evaluated by means of an in-vitro Fenton assay. Gibbs monolayer experiments indicated that polyphenols readily interacted with lipid films. Also, the exclusion surface pressure of the polyphenols in different monolayers indicated that this interaction is dependent on the composition and phase state of the membrane. Furthermore, fluorescence anisotropy studies showed that polyphenols were able to modulate me mbrane viscosity depending on their composition and phase state. However, polyphenols were unable to inhibit lipid oxidation induced by Fenton reaction with the exception of resveratrol. The results presented in this work indicate that polyphenols are able to closely interact with biological membranes but the antioxidant activity is not related to their membrane affinities.