INVESTIGADORES
MINAHK Carlos Javier
congresos y reuniones científicas
Título:
Polyphenols-membrane interaction and protection against lipid oxidation. A biophysical study
Autor/es:
DE ATHAYDE MONCORVO, A; MINAHK, C; ACUÑA, L; DUPUY, F
Lugar:
Rosario
Reunión:
Congreso; L Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; 2014
Institución organizadora:
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Resumen:
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Polyphenols are a
heterogeneous group of secondary metabolites, characterized by
the presence of two or more phenolic groups. The
characterization of the interaction of polyphenols with membranes may
provide insights on the mechanism of action of the antioxidant and
potentially protective activity of polyphenols on biological
membranes. The aim of the present work was to determine the membrane
interaction of resveratrol, naringenin, epigallocatechin gallate and
enterodiol by means of biophysica l techniques in model systems.
Also, the extent of lipid oxidation in the presence of polyphenols
was evaluated by means of an in-vitro Fenton assay. Gibbs monolayer
experiments indicated that polyphenols readily interacted with
lipid films. Also, the exclusion surface pressure of the
polyphenols in different monolayers indicated that this interaction
is dependent on the composition and phase state of the membrane.
Furthermore, fluorescence anisotropy studies showed that
polyphenols were able to modulate me mbrane viscosity
depending on their composition and phase state. However, polyphenols
were unable to inhibit lipid oxidation induced by Fenton reaction
with the exception of resveratrol. The results presented in this work
indicate that polyphenols are able to closely interact with
biological membranes but the antioxidant activity is not
related to their membrane affinities.