INVESTIGADORES
MINAHK Carlos Javier
congresos y reuniones científicas
Título:
Protoplasts from Listeria monocytogenes 7 resistant mutant cannot interact with enterocin CRL35
Autor/es:
MASIAS, E; DUPUY, F; MINAHK, C; BELLOMIO, A; CILLI, E; ACUÑA, L
Lugar:
Rosario
Reunión:
Congreso; L Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; 2014
Institución organizadora:
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Resumen:
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Enterocin CRL35 is
a pediocin-like bacteriocin produced by Enterococcus mundtii
CRL35. It is active against the food-borne pathogen Listeria
monocytogenes. To further study its mechanism of action, two
resistant Listeria variants were isolated from sensitive cells.
Once confirmed the identity of the mutant strains by rep-PCR
and 16S ribosomal RNA gene sequencing, its phenotype
was compared with the sensitive parental strain, Listeria monocytogenes 7.
The growth of the resistant mutants was slower as compared
to the parental strain with lower glucose consumption and a
lower medium acidification, showing differences in their metabolisms.
On the other hand, even though enterocin CRL35 was able to bind to
both sensitive and resistant cells with similar affinity, it was
unable to interact with the protoplasts of one of the resistant
mutant, as it was revealed by fluorescence polarization. The lackof membrane
association in protoplasts strongly suggests that major changes in
the plasma membrane of that mutant may take place. Therefore, a
complete analysis of the membrane was carried out in order
to fully describe the enterocin interaction. The present work
shows novel information about the enterocin CRL35 requirements
for membrane binding, which is believed to be the key step in the
mechanism of action