Resveratrol modulates ATPase activity of cholesterol transporter ABCG1

DE ATHAYDE MONCORVO, A; MORERO, R; MINAHK, C

Potrero de los Funes, San Luis

Congreso; XLVII Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; 2011

Sociedad Argentina de Investigación en Bioquímica y Biología Molecular

Atherosclerosis is an inflammatory disease of the blood vessels that constitutes the primary cause of cardiovascular disease. High density lipoproteins have a pronounced protective effect against atherosclerosis since they are involved in the transport of cholesterol from peripheral tissues to the liver. Stimulation of the activity of cholesterol transport proteins involved in this process was suggested as a promising strategy for the treatment and prevention of atherosclerosis. ABCG1 is one of the key transporters involved in the formation of HDL. In this work, human ABCG1 gene was sub-cloned into the pCHH10m3C shuttle vector. Then Saccharomyces cerevisiae BJ2168 was transformed using a variation of the classic lithium acetate protocol. The transporter was purified from the membrane fraction by affinity chromatography. Purified ABCG1 kept its ATPase activity in detergent micells as well as after reconstitution into liposomes. Finally, the modulation of such activity was evaluated upon addition of resveratrol; and it was concluded that this polyphenol was able to enhance ABCG1 ATPase activity by aproximately 50% at 0.1 mM. This work provides the foundation for the future implementation of this strategy in the evaluation of compounds on ABCG1 activity, enabling a quick and reliable identification of potentially active substances by in vitro activity assays.