Chemokines mediated interaction between maternal monocytes and trophoblast cells is differentially induced by PAMPS stimuli

E. GRASSO; D. PAPARINI; L. FRACCAROLI; V. HAUK; G. SALAMONE; C. PÉREZ LEIRÓS; R. RAMHORST

Congreso; V SLIMP - IV LASRI Meeting 2013; 2013

Background: We investigated chemokine/chemokine receptor modula- tion on monocytes during their interaction with trophoblast cells; and whether PAMPs stimuli modulate their functional prole and migratory properties. Methods: Swan 71 cells were cultured with monocytes isolated from fertile women in transwell systems that allow or not monocyte migration. Trophoblast cells were challenged with LPS, PGN or poly[I:C]. Supernatants and cells were recovered for ELISA, FACS and RT-PCR analysis (Protocol approved Academy of Medicine Ethics Committee). Results: Maternal CD14þ cells increased IL-10 while decreased IL-1 and TNFa production after the interaction with Swan-71 cells. Monocyte migration toward trophoblast cells was not increased in the presence of LPS or PGN as we observed with poly[I:C], and was accompanied by a decreased expression of CCR1, CCR5, CXCL-8 and CCL5. Trophoblast cells did not increase chemokine expression after cultured with monocytes in the presence of pathological stimuli. Conclusion: Trophoblast might induce an alternative macrophage prole and restrain maternal monocyte recruitment under pathological stimulation.