INVESTIGADORES
FRACCAROLI Laura Virginia
congresos y reuniones científicas
Título:
VIP modulates the expression of RANTES and the recruitment of iTregs on endometrial stromal cells
Autor/es:
E. GRASSO; L. FRACCAROLI; V. HAUK; G. MOR; C. PÉREZ LEIRÓS; R. RAMHORST
Reunión:
Congreso; 3rd Latin-American Symposium of Reproductive Immunology and 1st Meeting of the Latin American Chapter of the American Society for Reproductive Immunology; 2011
Resumen:
Introduction The generation and maintenance of the maternal-placental interface involves the modulation of chemokines and the recruitment of different leukocyte populations such as inducible regulatory T cells (iTregs). The vasoactive intestinal peptide (VIP) is produce by trophoblast cells and modulates the maternal immune response towards a tolerogenic profile. Here we evaluate the VIP/VPAC system and its involvement in the modulation of the production of chemokines and the recruitment of iTregs in endometrial cells. Materials and methods The system VIP/VPAC and the expression of chemokines was evaluated in HESC (human endometrial stromal cells) cell line by PCR under different combination of VIP (10-7M), progesterone (10-6M) and LPS (0,1ug/ml). Migration assay were performed using transwell system with iTregs differentiated in vitro with IL-2 and TGF-b from CD4 naïve leukocytes and evaluated by flow cytometry. Results In HESC cells, VIP and its receptor VPAC1 are expressed constitutively and progesterone increased the expression of VIP. The stimuli with LPS increased the expression of IL-8, MCP-1 and RANTES. This last one, was even more increased when was co-stimulated with VIP. When we stimulated the endogenous expression of VIP using progesterone, the expression of RANTES was increased and this effect was prevented with an antagonist of VIP. When we evaluated the ability of HESC to attract iTregs under different stimuli, we observed that iTregs were selectively attracted and it was increased by LPS. Conclusion We conclude that VIP may have an active role in recruiting iTregs to endometrial stromal cells by modulating the expression of RANTES.