Trophoblast cells recruit maternal Monocytes and modulate the chemokines and their receptors expression-profile under pathological conditions

E. GRASSO; L. FRACCAROLI; V. HAUK; F. BAUDOU; C. PÉREZ LEIRÓS; R. RAMHORST

Buenos Aires

Congreso; First French-Argentine Immunology Congress-LVIII Reunión Anual de la Sociedad Argentina de Inmunología; 2010

Sociedad Argentina de Inmunología

Macrophages constitute 20-30% of decidual immune cells and they coordinate the defense against pathogens, wound healing and tolerance induction against paternal antigens. Here, we study whether the maternal monocytes (MO) are attracting toward the trophoblat cells under physiological and pathological conditions. First, we performed migration assay with 8um transwell system using maternal PBMC in the upper compartment and trophoblat cells (Swan-71 cells line) in the lower compartment. The FACS analysis showed that trophoblast attracts CD14+ cells and this frequency significantly increases in the presence of Poli:IC (p0.05 student T-test). In order to discriminate the chemokines/receptors involved we performed non-migrating 0.4um transwell system seeding maternal MO (purified by CD14-magnetic beads) in the upper compartment and Swan cells in the lower. After 24 hours, both populations were recovered and the expression of MCP-1, IL-8, RANTES and their receptors (CCR1, CCR3 and CCR5) evaluated by RT-PCR. While, MO increased almost 1,5 fold CCR1, CCR3, and CCR5 expression, Swan cells increased MCP-1 and IL-8 expression after the dialogue. When these cultures were performed in the presence of LPS or PGN, MO reduced the expression of all receptors. However, the treatment with Poli:IC, which simulates a viral infection, did not decrease the receptors expression and increased the levels of MCP-1 and RANTES produced by Swan cells. These modulations were not observed in MO without trophoblast dialogue. In conclusion, during the MO-trophobalst dialogue, the MO are more sensitive to chemokines produced by trophoblat cells. In the presence of bacterial antigens, chemokines receptors are reduce making MO less sensitive to the recruitment, however in a viral infection, MO recruitment is potentiated by chemokines produced by trophoblast cells and this effect could be related with the proabortive effect of viral infections.