Modulation of maternal LIF producers T cells by trophoblast interaction and paternal antigens

L. FRACCAROLI; E. GRASSO; E. ZEITLER; E. LOMBARDI; S. GOGORZA; J. ETCHEPAREBORDA; C. NAGLE; M. CORTELEZZI; C. PÉREZ LEIRÓS; R. RAMHORST

AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY

WILEY-BLACKWELL PUBLISHING, INC

Año: 2011 vol. 65 p. 133 - 145

8755-8920

Problem Fetal implantation enhances the production of essential growth factors such as LIF (leukaemia inhibitory factor), hence we investigated the contribution of maternal CD4 cells, activated by paternal or trophoblast antigens and its modulation by VIP (vasoactive intestinal peptide) and progesterone. Method of study We performed co cultures of trophoblast cells (Swan-71 cell line) or paternal antigens and PBMCs from patients with recurrent spontaneous abortions (RSA) and fertile women. Result Fertile-CD4+LIF+ cells were increased by VIP and progesterone in response to paternal and trophoblast antigens. Also MMP-9 activity was decreased and pSTAT3/STAT3 ratio was increased. RSA patients have decreased levels of LIF expression which could not be modulated by VIP and progesterone and displayed a reduced number of endometrial infiltrated CD4+LIF+ cells compared with fertile women. Conclusion The decrease of CD4+LIF+ cells in RSA patients could be related with the exacerbated inflammatory response observed in the maternal-fetal dialogue model.