INSTITUTO DE INVESTIGACIONES BIOTECNOLOGICAS
Unidad Ejecutora - UE
congresos y reuniones científicas
rRNA operon copy-number impact on growth rate in Brucella and Bradyrhizobium.
LEILA BARTROLÍ; ELÍAS MONGIARDINI; ALEXIS ROMANÍ; DIEGO J COMERCI; IAN MEDICI; CAROLA CAMPANELLI; ALFONSO SOLER BISTUÉ
Congreso; Reunión Anual de Sociedades de Biociencias; 2020
SAIC SAI SAFIS
Growth rate (GR) varies widely among bacterial species. Comparative genomics suggest that the structure of the chromosome contributes to determine GR. In particular, bacteria bearing a high number of ribosomal operons (rrn) display higher GRs. The rrn number varies widely across bacteria ranging from 1 to 16 copies, with an average of 7 copies per genome. α- proteobacteria from Bradyrhizobia and Brucella genera are capable of interacting, either symbiotically or pathogenically, with hosts. Their low GR complicates their study. Close examination of complete genomes within these group of slow growers, shows that these clades bear 1 to 3 rrn. To test the links between their growth and ploidy of ribosomal RNA genes, we compared GRs of different isolates and we modified the rrn content within the studied groups. On one hand, we compared growth through manual and automated growth curves, of completely sequenced strains of the Bradyrhizobium genus: B. diazoeficiens USDA110 and B. diazoefficiens USDA122, with one rrn and B. japonicum E109 and B. japonicum USDA6T that have 2 rrns. We observed that strains bearing 2 rrn grew faster (p