IMMUNOMODULATORY EFFECT OF U-OMP19 IN A PAPAIN-INDUCED ALLERGY MODEL

PUEBLAS CASTRO, CELESTE; PASQUEVICH, KARINA A.; CORIA, LORENA M.; CASSATARO, JULIANA; BRUNO, LAURA

Congreso; Reunión Anual de Sociedades de Biociencias SAIC. SAI. SAFIS 2020; 2020

SAIC.SAI. SAFIS

Allergies are T helper 2 immune responses mediated by IgE, inducedby an antigen called allergen. Many allergens are proteases;thus, allergy models were developed using cysteine proteases likepapain.At our laboratory we had demonstrated the potential of U-Omp19as an adjuvant in vaccine formulations against infectious diseasesinducing a Th1 response. Therefore, the main objective of this workwas to evaluate its potential to modulate allergic responses.To this end an allergy model induced by papain was performed atour laboratory. Mice (n=5/group) were subcutaneously immunizedwith OVA, OVA+papain or OVA+papain+U-Omp19. The antibody responsewas assessed by analyzing anti-OVA IgE, IgG1 and IgG2ain serum by indirect ELISA. The specific cellular immune responsewas also measured, analyzing the levels of cytokines associatedwith a Th2 response (IL-5 and IL-13) in OVA-stimulated splenocytesupernatants by ELISA.Our results indicate that the allergic response was induced whenmice were immunized with OVA plus papain. In this group, we havefound a significant increase in anti-OVA IgE levels (p